The most significant current metabolomics challenge is the
identification and interpretation of vast datasets despite the fact that
numerous mass spectrometry platforms have evolved. To address this challenge,
IROA TechnologiesLLChas developed IROA TruQuant IQQ. TruQuant IQQ utilizes a set of
complexinternal standards for biomarker discovery and reproducibility. IROA
methodologies eliminate analytical variability, noise, and ion suppression and
thus, make it easy to achieve clean, accurate and reproducible datasets.
IROA TruQuant IQQ workflow – How this works
Currently, most metabolomics datasets can only be compared
with same-day-runsamples. While this may be adequate for small research
projects, a more reliable workflow is required for clinically relevant
metabolomics projects to assure accurate identification, completely
reproducible quantitation and performance criteria for instrumentation. In order to make this possible, advanced
metabolic profiling tool are required.
The most significant current metabolomics challenge is the
identification and interpretation of vast datasets despite the fact that
numerous mass spectrometry platforms have evolved. To address this challenge,
IROA TechnologiesLLChas developed IROA TruQuant IQQ. TruQuant IQQ utilizes a set of
complexinternal standards for biomarker discovery and reproducibility. IROA
methodologies eliminate analytical variability, noise, and ion suppression and
thus, make it easy to achieve clean, accurate and reproducible datasets.
IROA TruQuant IQQ workflow – How this works
Currently, most metabolomics datasets can only be compared
with same-day-runsamples. While this may be adequate for small research
projects, a more reliable workflow is required for clinically relevant
metabolomics projects to assure accurate identification, completely
reproducible quantitation and performance criteria for instrumentation. In order to make this possible, advanced
metabolic profiling tool are required.
IROA TruQuant IQQ, an advanced metabolic profiling tool,
includes a set of complex internal standards for biomarker discovery and
reproducibility. These internal standardscorrect for ionization
inefficiencies/matrix effects in order to produce suppression-corrected data
and provide cross-platform quantitative reproducibility together with validated
compound identification and consistent instrument QA/QC.
The TruQuant IQQ workflow contains two sets of complex
metabolite C13-labeledLong-Term internal standards can help you to overcome the
cross-platform normalization of measurements using the same or different
analytical methodologies. These internal standards provide the means for data
standardization while also providing high-quality data, quality assurance,
compound identification, and quantitation.
The IROA TruQuant IQQ workflow includesa carefully formulated
long-term reference standard (IROA-LTRS) that contains over 500 compounds
labeled with 95%- and 5% C13 (and mixed 1:1). Why95%/5%? These unique
metabolite signatures in the LTRS are computationally easy to find and enables
a software solution to assure completely
verified compound identification and also provide a daily measure of
instrumentation performance.
The second set of IROA Internal Standards (IROA-IS) has the
same chemical profile as the IROA-LTRS(based on the same concentration of
95%-C13 only) andandthis acts as an internal standard (spiked into experimental
samples) to locate corresponding natural abundance isotopomers in any sample.
Contact IROA Technologies, LLC
Contact IROA Technologies atinfo@iroatech.com or to learn
more visit iroatech.com.
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