Metabolic profiling tools using full metabolic labeling have long been used to simplify the metabolomics research process. Conventionally, in-vitro metabolite stable isotope labeling has been carried out by incorporating 99% 13C by feeding cells with isotopically labeled media. Precise identification, and reproducible quantitation, using these systems can be challenging.
Why adopt 95% and 5% 13C labeling instead?
The unique mass spectral patterns of molecules that are 5% 13C and 95% 13C-labeled have the advantage over traditional methods that use no label or use heavy (99%) and light (unlabeled, natural abundance).
- 95% and 5% 13C labeling gives rise to unique and mathematically calculable isotopic patterns, clearly differentiated from natural abundance non-biological artifacts and noise.
- The number of carbons for each and every metabolite can be determined; carbon number and mass together greatly confine the number of probable molecular formulae.
- Sample-to-sample variance is overcome and ion suppressioncan be removed.
- IROA ClusterFinder software tool easily characterizes peaks (as either artifacts, control or experimental compounds), removes artifacts, performs peak correlation analysis to associate adducts with their fragments, calculates carbon number and molecular formula, quantitates the ratio of control to experimental, removes suppression and normalizes each dataset.
Contact “IROA Technologies LLC” for more information on these advanced tools (iroatech.com).
Comments
Post a Comment